Thus, the time course of aggregation can be monitored with ThT. Thioflavin T is a fluorescence dye that becomes fluorescent when bound to aggregated PrP I. Each dilution series is replicated several times and each sample of this series is applied to vigorous shaking typically in a microplate reader capable of detecting Thioflavin T (ThT) fluorescence. The dilution series is typically performed on a logarithmic scale while keeping the concentration of diluent (PrP C solution) constant. cerebrospinal fluid) supposed to contain infectious PrP I is diluted with native-conformation-PrP C. In an RT-QuIC assay a liquid body sample (e.g. A short-hand notation for prion protein that is often used is PrP. proteins that can induce conformational changes in other proteins and lead to diseases like Creutzfeldt-Jakob disease. ![]() These assays are typically used to detect small amounts of prions, i.e. The first time I came across the term Spearman-Kärber was with the analysis of RT-QuIC. A non-parametric analysis to determine the ED50, SD50 or similar metrics
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